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November 2013

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Confocal Microscopy List <[log in to unmask]>
Subject:
Silver enhanced autoradiography / dark field under confocal ?
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Renato Mortara <[log in to unmask]>
Date:
Mon, 25 Nov 2013 08:29:45 -0200
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Dear friends,



A colleague is trying to image mouse brain sections that were radioactively
labeled (I guess 35S-Methionine) then, immersed in a photographic emulsion
to develop particular sites.



The sections were then counter stained with toluidine or similar (bluish)
histological dye. The final result ('bright spots, easily seen under a 20x
objective) can be visualized under dark field microscopy.



I wonder if anyone has been able to image through some kind of reflection
mode under confocal microscopy.



Thanks !



Renato Mortara



Dr. Renato Arruda Mortara

Parasitology Division

Escola Paulista de Medicina - UNIFESP

Rua Botucatu,  862 6th floor

04023-062

São Paulo SP Brazil

Phone: 55 11 55798306

www.ecb.epm.br/~ramortara

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