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Back in the early 1990s we used reflectance confocal to image in situ.  Also, enhanced immunogold and lead ATPase.  No one has asked for it recently, but even after a decade it must still work.

===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell:  914-309-3270   Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Renato Mortara
Sent: Monday, November 25, 2013 5:30 AM
To: [log in to unmask]
Subject: Silver enhanced autoradiography / dark field under confocal ?

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Dear friends,



A colleague is trying to image mouse brain sections that were radioactively labeled (I guess 35S-Methionine) then, immersed in a photographic emulsion to develop particular sites.



The sections were then counter stained with toluidine or similar (bluish) histological dye. The final result ('bright spots, easily seen under a 20x
objective) can be visualized under dark field microscopy.



I wonder if anyone has been able to image through some kind of reflection mode under confocal microscopy.



Thanks !



Renato Mortara



Dr. Renato Arruda Mortara

Parasitology Division

Escola Paulista de Medicina - UNIFESP

Rua Botucatu,  862 6th floor

04023-062

São Paulo SP Brazil

Phone: 55 11 55798306

www.ecb.epm.br/~ramortara

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