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Thanks for the links and answers! That helps.
We might also go for measuring with a spectrophotometer...
Cheers,
Oliver
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Oliver Biehlmaier, PhD
Head of Imaging Core Facility
Biozentrum (Kragenbau, Room G1054)
University of Basel
Klingelbergstrasse 50/70
4056 Basel
Switzerland
Office: +41 (61) 267 20 73
Lab: +41 (61) 267 22 50
Email: [log in to unmask]
http://www.biozentrum.unibas.ch/imcf
http://microscopynetwork.unibas.ch
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Am 05.11.2013 um 17:30 schrieb Chris Tully:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Oliver,
>
> I would highly recommend asking around among your local colleagues for someone who has a fluorescence spectrophotometer, and measure the curves yourself.
>
> Alternatively, I have read this article because I do not have access to it at the moment but according to Google it does have a UV-Vis absorbance spectrum.
>
> Chris
>
> On 11/5/2013 10:45 AM, Oliver Biehlmaier wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear listers,
>>
>> I have a question regarding Evans Blue:
>>
>> I have a user that is doing standard 3ch-labelings on a confocal microscope (DAPI, Alexa488, Alexa 568). Now she is having some sections from mice that have been injected with Evans Blue.
>> When imaging these new samples, she wants to use the same 3ch approach as before but she does not want to see any fluorescence from the Evans Blue.
>> I tried to find out what the excitation and emission spectrum of Evans Blue is but I could not find any clear information. E.g. one publication specifies the spectrum of Evans Blue as follows: excitation at 620 nm, emission at 680 nm (Saria A, Lundberg JM. Evans blue fluorescence: quantitative and morphological evaluation of vascular permeability in animal tissues. J Neurosci Methods. 1983 May;8(1):41-9. PubMed PMID: 6876872.) Others claim that it fluoresces with excitation peaks at 470 and 540 nm and that it has its emission peak at 680 nm. I tend towards the first spectrum but I did not find any other confirmation for it.
>>
>> Does anyone of you guys know the exact spectrum of that dye?
>> This would be great!
>>
>> Cheers,
>> Oliver
>>
>> ----------------------------------------------------------------
>>
>> Oliver Biehlmaier, PhD
>>
>> Head of Imaging Core Facility
>>
>> Biozentrum (Kragenbau, Room G1054)
>> University of Basel
>> Klingelbergstrasse 50/70
>> 4056 Basel
>> Switzerland
>>
>> Office: +41 (61) 267 20 73
>> Lab: +41 (61) 267 22 50
>> Email: [log in to unmask]
>> http://www.biozentrum.unibas.ch/imcf
>> http://microscopynetwork.unibas.ch
>> ----------------------------------------------------------------
>
>
> --
> *Chris Tully*
> Principal Consultant
> Image Incyte, LLC
> 240-475-9753
>
>
> Image Incyte, LLC
>
> <http:%5C%5Cwww.ImageIncyte.com> [log in to unmask] <mailto:[log in to unmask]>
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