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See:

High-resolution multiphoton cryomicroscopy. 
<http://www.ncbi.nlm.nih.gov/pubmed/23867337>

König K, Uchugonova A, *Breunig* HG.

Methods. 2013 Jul 16. doi:pii: S1046-2023(13)00255-7. 
10.1016/j.ymeth.2013.07.006. [Epub ahead of print]

PMID:
    23867337


Multiphoton imaging of freezing and heating effects in plant leaves. 
<http://www.ncbi.nlm.nih.gov/pubmed/22987831>

*Breunig* HG, Tümer F, König K.

J Biophotonics. 2013 Aug;6(8):622-30. doi: 10.1002/jbio.201200093. Epub 
2012 Sep 14.

PMID:
    22987831



Karsten Konig is a founder of JenLab -
http://www.jenlab.de/2PM-Cryo.133.0.html?&L=en 
<http://www.jenlab.de/2PM-Cryo.133.0.html?&L=en>

The manufacturer of the freezing unit is described in the papers above. 
Would work for 1-photon fluorescence as well.


    CryoSTED microscopy - a new spectroscopic approach for improving the
    resolution of STED microscopy using low temperature

    Giske, Arnold
    http://archiv.ub.uni-heidelberg.de/volltextserver/7969/


When cold enough, I believe the shape of the fluorescence spectra become 
much more structured, which could have advantages for multiplexing. See 
Giske dissertation for more on cold photophysics.

Enjoy,

George


On 11/29/2013 8:46 AM, Rickman, Colin wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I have been searching online looking for papers that have studied the fluorescent properties of fluorescent proteins and organic dyes. I haven’t managed to find much and wondered if anybody on this list has tried to do these experiments or is aware of any publications.
>
> Regards
>
> Colin
>
>
> Dr Colin Rickman
> Institute of Biological Chemistry, Biophysics and Bioengineering
> School of Engineering and Physical Sciences
> Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: +44 131 4514193 (Office)
>
> http://www.ib3.eps.hw.ac.uk
> http://www.esric.org
>
>
>
>
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-- 



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/