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Dear all,

If Evans Blue peaks at ~ 680 nm I was probably not looking at it with the right optics on our 2-P scope. Forget what I said about Evans Blue not visible under 2-P.

Quoc Thang Nguyen
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On Nov 6, 2013, at 7:13 AM, Jean-Marie Vanderwinden wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> 
> Dear,
> 
> no personal experience but see:
> 
> Li JL, Goh CC, Keeble JL, Qin JS, Roediger B, Jain R, Wang Y, Chew WK, Weninger W, Ng LG. Intravital multiphoton imaging of immune responses in the mouse ear skin. Nat Protoc 2012;7:221-234
> http://www.ncbi.nlm.nih.gov/pubmed/22240584
> 
> p223 - quote
> 
> Table 1 | List of vessel-labeling agents.
> Vessel-labeling agentsAdvantagesDisadvantages
> Evans blue dyeInexpensive
> Bright
> Relatively nontoxic
> Good contrast over a wide range of excitation wavelengths (720-990 nm)
> Sharp emission peak at ~680 nm
> Obvious visual cue to the success of an injectionLeakage into mouse ear interstitium
> end quote
> 
> Sincerely.
> 
> JM
> 
> At 14:52 6/11/2013, you wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> On 05.11.2013 21:47, Quoc Thang Nguyen wrote:
>>> 
>>> Also, I could not get any two-photon images with Evans Blue with the same specimens, owing possibly to the symmetrical structure of Evans Blue that would preclude multiphoton excitation.
>> 
>> Multi-photon excitation worked nice on our system, with fluorescence in the 665 nm channel. So it works in principle. I seem to remember that excitation was around 830 nm but am not sure about it.
>> 
>> Steffen
>> 
>> --
>> ------------------------------------------------------------
>> Steffen Dietzel, PD Dr. rer. nat
>> Ludwig-Maximilians-Universität München
>> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
>> Head of light microscopy
>> 
>> Mail room:
>> Marchioninistr. 15, D-81377 München
>> 
>> Building location:
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> 
> Jean-Marie.
> 
> Jean-Marie Vanderwinden, M.D., Ph.D.
> Directeur de Recherche F.N.R.S. / Research Director, National Fund for Scientific Research (Belgium)
> 
> Université Libre de Bruxelles (ULB)
> 
> ULB Neuroscience Institute - Neurophysiology lab http://www.ulb.ac.be/medecine/neurophy/
> 
> Light Microscopy Facility http://limif.ulb.ac.be/
> 
> Postal address:
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