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Vetbond works somewhat; I've seen it used to tack down dorsal root, etc. We
usually use nets to hold tissue down in a tiny perfusion chamber though, as
this keeps it happy (or at least less sad...) during imaging.


On Mon, Nov 11, 2013 at 6:23 AM, MORONE Diego RIC <
[log in to unmask]> wrote:

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> Dear Jean-Pierre,
>
> I use a drop of 100% glycerol to mount fresh thick tissues, it should work
> with thin tissues. You could also try vetbond surgical glue
>
> Best
> Diego
>
>
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> -----Original Message-----
> From: Jean-Pierre CLAMME [mailto:[log in to unmask]]
> Sent: venerdì 8 novembre 2013 20:11
> Subject: Mounting non fixed tissue ?
>
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> Hi,
>
> I was wondering if anyone knows about the possibility of mounting thin
> tissue sections without fixing the tissue. What would be a protocol for
> such samples ?
> All the protocol I've found involve fixing.
>
> Thank you.
>
> JP
>