CONFOCALMICROSCOPY Archives

February 2014

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Condense Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Content-Type:
text/plain; charset=ISO-8859-1
Sender:
Confocal Microscopy List <[log in to unmask]>
Subject:
Re: MITOCHINDRIA
From:
Manish Kumar <[log in to unmask]>
Date:
Tue, 25 Feb 2014 16:36:04 +0530
In-Reply-To:
<[log in to unmask]>
MIME-Version:
1.0
Reply-To:
Confocal Microscopy List <[log in to unmask]>
Parts/Attachments:
text/plain (208 lines) 
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

you can meet me at Mathura road campus.


On Tue, Feb 25, 2014 at 4:16 PM, Chaitali Banerjee <
[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Manish
> I have used both control and treated sample. I am using Zeiss automated
> microscope and using 100x. Can u please tell me in which campus you are
> working-mall road or mathura road. I am in north campus,Delhi. I can come
> to meet u then.
> Regards
> Chaitali
>
>
> On Tue, Feb 25, 2014 at 3:14 PM, Manish Kumar <[log in to unmask]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi Chaitali,
> > Did you perform the control sample along with your experimental sample,
> > which microscope/ software you used for colocalization analysis?
> >
> > Rgds
> > Manish
> > Imaging Facility Manager
> > IGIB, New Delhi
> >
> >
> > On Tue, Feb 25, 2014 at 11:13 AM, Chaitali Banerjee <
> > [log in to unmask]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > I am a PhD stuent from University of Dlehi, India. Currently I am
> > > performing some experiments with mito tracker and ER tracker and was
> > > studying mitochondrial movement towards ER. When i tried co
> localization
> > i
> > > was nt happy with my results. I am using 10 nm of mitotracker & er
> > > tracker.When
> > > i looked into literature i got sm pictures like what i hv got,
> > > but I found that they further digitally magnified the images several
> > fold.
> > > I tried with the photoshop & imagej to get the best magnificatn. But
> > still
> > > nt much convincd. Any suggestn please.
> > > Regards
> > > Chaitali Banerjee
> > > PhD student
> > > Department of Zoology
> > > University of Delhi
> > > India
> > >
> > >
> > > On Mon, Feb 24, 2014 at 8:45 PM, Glen MacDonald <
> > [log in to unmask]
> > > >wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > *****
> > > >
> > > > Given the gmail accounts and lack of affiliation, are you and
> Chaitali
> > > > working together?  Are you looking for signal or resolution?  Do you
> > > have a
> > > > suitable camera?  An overly corrected objective, low NA or phase
> > > objectives
> > > > will all reduce signal.  Mitotrackers in zebrafish can be detected in
> > > > widefield or confocal with a 40X/.75 (or 20X + 2X zoom) air lens, and
> > > > easily recorded with NA 1.0-1.2.  I hope you are not trying to look
> > > through
> > > > a plastic culture dish.  Do you have any experience with live
> imaging,
> > > > microscopy or dye loading? Have you looked in the literature?   There
> > are
> > > > many papers using Mitotrackers, and in zebrafish.
> > > >
> > > > Providing a description of your instrumentation, experimental intent
> > and
> > > > sample preparations when  you ask for assistance will avoid a game of
> > "20
> > > > Questions".
> > > >
> > > > Mitotrackers require testing for concentration, incubation times,
> > > > temperature, etc. before embarking on any experiment but the
> literature
> > > > will provide starting points.  Overloading can reduce intensity (by
> > > > disrupting mitochondrial function), and is a common beginner's
> mistake.
> > > >  try 25 nM to 100 nM, 20-30 min. or shorter.  MT Red is brighter, use
> > no
> > > > more than 25 nM, Deep Red behaves like MT Green, bt possibly a bit
> > > voltage
> > > > sensitive.
> > > >
> > > > Digital zoom will not improve signal, it sometimes helps with
> > visibility
> > > > on the screen.
> > > >
> > > > Glen MacDonald
> > > >         Core for Communication Research
> > > > Virginia Merrill Bloedel Hearing Research Center
> > > >         Cellular Morphology Core
> > > > Center on Human Development and Disability
> > > > Box 357923
> > > > University of Washington
> > > > Seattle, WA 98195-7923  USA
> > > > (206) 616-4156
> > > > [log in to unmask]
> > > >
> > > >
> > > >
> > > >
> > > >
> > > >
> > > >
> > > > On Feb 24, 2014, at 3:06 AM, Amandeep Walia <[log in to unmask]>
> wrote:
> > > >
> > > > > *****
> > > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > > *****
> > > > >
> > > > > Dear even i am trying to but getting nothing
> > > > >
> > > > >
> > > > >
> > > > > On Mon, Feb 24, 2014 at 4:14 PM, Chaitali Banerjee <
> > > > > [log in to unmask]> wrote:
> > > > >
> > > > >> *****
> > > > >> To join, leave or search the confocal microscopy listserv, go to:
> > > > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > >> *****
> > > > >>
> > > > >> I am also trying to localize mitochondria in fish macrophages
> using
> > > > >> mitotracker. What I am getting is greenish tinge of mitotracker
> at x
> > > 100
> > > > >> objective. Can u please suggest me if there is some software for
> > > digital
> > > > >> zooming
> > > > >> Regards
> > > > >> Chaitali
> > > > >>
> > > > >>
> > > > >> On Mon, Feb 24, 2014 at 3:59 PM, Sergey Tauger <[log in to unmask]
> >
> > > > wrote:
> > > > >>
> > > > >>> *****
> > > > >>> To join, leave or search the confocal microscopy listserv, go to:
> > > > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > >>> *****
> > > > >>>
> > > > >>> Could you please tell what exactly you are going to image and
> what
> > is
> > > > the
> > > > >>> label?
> > > > >>> Are you going to perform some quantitative imgaing or just make
> > sure
> > > > that
> > > > >>> your
> > > > >>> sample really contains mitochondria? What is the cell type?
> > > > >>>
> > > > >>> Mitchondria are well visible for 1.2 NA objective (TMRE staining,
> > > > >>> lymphocytes) and
> > > > >>> qualitatively visible while using NA 1 objective if cells are
> > > spreaded
> > > > >>> (MitoTracker
> > > > >>> Green, Vero cells)
> > > > >>>
> > > > >>> Best
> > > > >>> Sergey Tauger
> > > > >>> PhD student
> > > > >>> Cell motility lab.
> > > > >>> Dept. of Biology
> > > > >>> Moscow State University
> > > > >>>
> > > > >>
> > > >
> > >
> >
>

ATOM RSS1 RSS2