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Dear even i am trying to but getting nothing



On Mon, Feb 24, 2014 at 4:14 PM, Chaitali Banerjee <
[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I am also trying to localize mitochondria in fish macrophages using
> mitotracker. What I am getting is greenish tinge of mitotracker at x 100
> objective. Can u please suggest me if there is some software for digital
> zooming
> Regards
> Chaitali
>
>
> On Mon, Feb 24, 2014 at 3:59 PM, Sergey Tauger <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Could you please tell what exactly you are going to image and what is the
> > label?
> > Are you going to perform some quantitative imgaing or just make sure that
> > your
> > sample really contains mitochondria? What is the cell type?
> >
> > Mitchondria are well visible for 1.2 NA objective (TMRE staining,
> > lymphocytes) and
> > qualitatively visible while using NA 1 objective if cells are spreaded
> > (MitoTracker
> > Green, Vero cells)
> >
> > Best
> > Sergey Tauger
> > PhD student
> > Cell motility lab.
> > Dept. of Biology
> > Moscow State University
> >
>