CONFOCALMICROSCOPY Archives

February 2014

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: MITOCHINDRIA
From:
Diego De Stefani <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 24 Feb 2014 14:55:36 +0100
Content-Type:
text/plain
Parts/Attachments:
text/plain (88 lines) 
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You don't need a specific objective to see mitochondria. You first need a
good staining. Best results are achieved with mitochondrial targeted FPs or
through immunofluorescence of mitochondrial proteins (TOM20, TOM40, HSP60
and many others). Otherwise,  if you want to use mitotracker dyes, you need
to set up dye concentration and loading time to obtain good results.

Diego De Stefani, PhD
Department of Biomedical Sciences
University of Padova
Il 24/feb/2014 13:53 "Chaitali Banerjee" <[log in to unmask]>
ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Anyone please help
>
>
> On Mon, Feb 24, 2014 at 4:36 PM, Amandeep Walia <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear even i am trying to but getting nothing
> >
> >
> >
> > On Mon, Feb 24, 2014 at 4:14 PM, Chaitali Banerjee <
> > [log in to unmask]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > I am also trying to localize mitochondria in fish macrophages using
> > > mitotracker. What I am getting is greenish tinge of mitotracker at x
> 100
> > > objective. Can u please suggest me if there is some software for
> digital
> > > zooming
> > > Regards
> > > Chaitali
> > >
> > >
> > > On Mon, Feb 24, 2014 at 3:59 PM, Sergey Tauger <[log in to unmask]>
> > wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > *****
> > > >
> > > > Could you please tell what exactly you are going to image and what is
> > the
> > > > label?
> > > > Are you going to perform some quantitative imgaing or just make sure
> > that
> > > > your
> > > > sample really contains mitochondria? What is the cell type?
> > > >
> > > > Mitchondria are well visible for 1.2 NA objective (TMRE staining,
> > > > lymphocytes) and
> > > > qualitatively visible while using NA 1 objective if cells are
> spreaded
> > > > (MitoTracker
> > > > Green, Vero cells)
> > > >
> > > > Best
> > > > Sergey Tauger
> > > > PhD student
> > > > Cell motility lab.
> > > > Dept. of Biology
> > > > Moscow State University
> > > >
> > >
> >
>

ATOM RSS1 RSS2