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Hello All,

Chromatic aberration is an artifact produced by differing wavelengths passing through a medium with a refractive index, and that n affects different wavelengths differently. 

In the example of a chromatically corrected objective, multiple wavelengths pass through a very specific combination of lenses to reduce the artifact/mis-alignment. 

When imaging a sample, say a monolayer or a tissue sample, the excitation (and emission for that matter) wavelengths pass through multiple n's.  Will this induce chromatic aberration in the emission pathway?

How is this corrected for?  In the objective again?  Or is it not necessary, or insignificant within the realm of light microscopy resolution. If not necessary for "standard" light microscopy, what about super res?

Thank you for explaining. 

Best,

Gary


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