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In addition to dental cement, I've been using rubber cement, which works
well and is cheap ('Fixogum' from Marabu with a pipette tip - no commercial
interest).
I generally advise against nail varnish, due to its strong autofluorescence
etc:

Fixation, mounting and sealing with nail polish of cell specimens lead to
incorrect FRET measurements using acceptor photobleaching.
Rodighiero S, Bazzini C, Ritter M, Fürst J, Botta G, Meyer G, Paulmichl M.
Cell Physiol Biochem. 2008;21(5-6):489-98. doi: 10.1159/000129642. Epub
2008 Apr 24.

_____________________________________
Philippe Laissue, PhD, Bioimaging Manager
School of Biological Sciences, Room 4.17
University of Essex, Colchester CO4 3SQ, UK
(0044) 01206 872246 / (0044) 07842 676 456
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privatewww.essex.ac.uk/~plaissue


On 27 February 2014 20:42, Christophe Leterrier <
[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Antonio,
>
> We have been using dental cement on fixed samples, and as stated it should
> be OK with live samples.
>
> For live samples I prefer using EMS Diasum silicone hybridization chamber:
> they exist in a lot different sizes and shapes to accommodate pretty much
> any type of coverslip. Some of them have an adhesive side that sticks on
> the slide, then you just need to fill the well (a few 10s-100s of µL will
> do) and gently press the coverslip, then aspirate the excess medium to seal
> the chamber
>
> It is easy to remove the coverslip for further processing, and the
> slide/chamber assembly is reusable. If you worry about the coverslip moving
> during imaging, we have been doing single particle tracking and
> super-resolution microscopy using these chambers without any noticeable
> problems compared to dental cement.
>
> Check here:
> http://www.emsdiasum.com/microscopy/products/preparation/hybridization.aspx
>
> For our 18mm round coverslips we use the one-side adhesive, 13mm wide,
> 0.6mm deep ref. 70-334-A:
>
> http://www.emsdiasum.com/microscopy/products/preparation/hybridization.aspx#70334
>
> Hope this helps,
>
> Christophe
>
> --
> Christophe Leterrier
> Researcher
> Axonal Domains Architecture Team
> CRN2M CNRS UMR 7286
> Aix Marseille University, France
>
>
>
>
> 2014-02-27 16:52 GMT+01:00 Antonio J Pereira <[log in to unmask]>:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> >
> > Hi all,
> >
> > For some purposes in live cell imaging we assemble a chamber with a
> > coverslip on top of a slide.
> > We use two halves of another coverslip to act as spacers.
> >
> > The  issue is the sealing. We've been using VALAP (a kind of wax) that we
> >  melt and then use to seal all the way through the periphery of the
> >  coverslip.
> >
> > It works, but it would be more convenient to have something that does not
> > have to be heated, and that would harden just by  contact with air.
> > A bit like the nail varnish used for fixed material ... but here we need
> > something which is biologically inert.
> >
> > Any suggestions? Thanks a lot.
> >
> > Antonio
> >
> >
> >
> > António Pereira
> > Chromosome Instability and Dynamics Group, Maiato lab
> >
> > IBMC - Institute for Molecular and Cell Biology
> > Porto- Portugal
> >
> >
> > António Pereira
> > IBMC - Instituto de Biologia Molecular e Celular
> > Grupo Instabilidade e Dinâmica Cromossómica
> >
> > Rua do Campo Alegre, 823
> > 4150-180 Porto
> > Portugal
> >
> > +351 22 607 49 00 (ext# 1620)
> > [log in to unmask]
> >
>