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February 2014

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From:
Shalin Mehta <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 28 Feb 2014 12:22:36 -0500
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Hello,

I have a question about the maximum usable field of view of commercial
objectives, if the only constraint is the mostly-flat field of view.
We would like to find a commercial low mag, high NA objective and
extract as much field of view as possible.

Is the usable field of view of an objective constrained by the field
number of the microscope? i.e., if the field number of the microscope
is 26 mm, is the diameter of the field of view constrained to 26mm/mag
of the objective?

Are there stops in the objective that enforce this specification?  If
we are to use a tube lens of same focal length as the commercial
microscope, but larger diameter, can we have a  field of view larger
than specified by the field number? Having a flat field of view will
be great, but slight imperfection is not a problem.

Thanks
Shalin

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