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Hi Shalin,

Maybe there is also a possibility to use a camera with less, bigger pixels
and use a "super-resolution" method : take several images with small
(sub-pixels) shifts and reconstruct an image with more, smaller pixels. I
think this was used in certain Zeiss cameras a few years ago (but I
couldn't find a link or reference just yet).

Best Regards,

Christophe


2014-02-28 18:22 GMT+01:00 Shalin Mehta <[log in to unmask]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I have a question about the maximum usable field of view of commercial
> objectives, if the only constraint is the mostly-flat field of view.
> We would like to find a commercial low mag, high NA objective and
> extract as much field of view as possible.
>
> Is the usable field of view of an objective constrained by the field
> number of the microscope? i.e., if the field number of the microscope
> is 26 mm, is the diameter of the field of view constrained to 26mm/mag
> of the objective?
>
> Are there stops in the objective that enforce this specification?  If
> we are to use a tube lens of same focal length as the commercial
> microscope, but larger diameter, can we have a  field of view larger
> than specified by the field number? Having a flat field of view will
> be great, but slight imperfection is not a problem.
>
> Thanks
> Shalin
>