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I've used a USB2.0 machine vision camera from Edmund Optics for histology
stains for around $800. It offers binning which lets you trade resolution
for speed:
http://www.edmundoptics.com/imaging/cameras/board-level-cameras/eo-usb-2-0-board-level-cameras/2947

ThorLabs offers an even cheaper camera which I have used for transmitted
light to good effect on another system:
http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4024

The main limit of these cameras is somewhat reduced sensitivity and low bit
depth, but for phase contrast it should be 'good enough'.

Craig Brideau


On Mon, Mar 31, 2014 at 9:47 AM, Jens-Bernhard Bosse <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
> I am looking for a budget camera (under 1K) for a microinjection/routine
> fluorescence rig. I will do the microinjection in phase contrast and need
> some fluorescence capability to check for microinjected fluorescent beads
> and fluorescent cells. I am not too sure if CMOS or CCD are better for the
> job. Port-wise USB, Firewire or GigE would be all fine. A higher frame rate
> capability (e.g. 25 fps) would be great as well as a resolution around one
> megapixel. Ideally it should be supported by either Matlab or Micromanager.
> Which model/company provides the best bang for the buck in your opinion?
>
> I would appreciate your suggestions!
>
> Thanks!
>
> Jens
>
> --------------------------------------------------------------
> Jens B. Bosse Ph.D.
> Enquist Lab
> Department of Molecular Biology
> and
> Princeton Neuroscience Institute
> Princeton University
> 301 Schultz Lab
> Washington Rd
> 08544 Princeton, NJ, USA
>
> Phone:  +1-609-258-4990
> Email:  [log in to unmask]
> Web:    http://molbio.princeton.edu/labs/enquist/
>