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Subject:	Re: Budget camera for microinjection/fluorescence microscopy
From:	David Baddeley <[log in to unmask]>
Reply To:	David Baddeley <[log in to unmask]>
Date:	Mon, 31 Mar 2014 12:09:58 -0700
Content-Type:	text/plain
Parts/Attachments:	text/plain (68 lines)

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If you're willing to push the budget to ~1100, we've been very happy with the Thorlabs high sensitivity CMOS (DCC1240M http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4024). The read noise is a bit high (estimated ~ 20-30 electrons), and somewhat non-uniform, but the QE is very good - we've even managed to get single molecule signals out of it.

cheers,
David
On Monday, 31 March 2014 2:41 PM, "Knecht, David" <[log in to unmask]> wrote:

Does this adapter support binning with any of the cameras? We find binning important for fluorescence and the adapter with the Sony XCD700 does not seem to support binning. Dave

On Mar 31, 2014, at 12:27 PM, Johannes Schindelin <[log in to unmask]<mailto:[log in to unmask]>> wrote:

Hi Jens,

On Mon, 31 Mar 2014, Jens-Bernhard Bosse wrote:

I am looking for a budget camera (under 1K) for a microinjection/routine
fluorescence rig. I will do the microinjection in phase contrast and
need some fluorescence capability to check for microinjected fluorescent
beads and fluorescent cells. I am not too sure if CMOS or CCD are better
for the job. Port-wise USB, Firewire or GigE would be all fine. A higher
frame rate capability (e.g. 25 fps) would be great as well as a
resolution around one megapixel. Ideally it should be supported by
either Matlab or Micromanager.

We dabbled with TheImagingSource cameras (supported by Micro-Manager, and
through Micro-Manager in Matlab, of course). The quality was decent, but I
would recommend the Firewire version because our USB model seemed to have
some problems with heat when switched on for eight hours or so. That might
be fixed in the meantime, though.

Another budget option might be IIDC machine vision cameras (supported by
Micro-Manager: http://www.micro-manager.org/wiki/Dc1394 -- limited Windows
support).

Having said that, in our project we were lucky to get ahold of an Orca
that was not currently in use, and the quality of the images is noticably
better. So we currently use neither of the cameras mentioned above.

Ciao,
Johannes

David Knecht, Ph.D.
Professor and Head of Core Microscopy Facility
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

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