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How many plex do you want to see?

Angelo M, Bendall SC, Finck R, Hale MB, Hitzman C, Borowsky AD, Levenson RM,Lowe JB, Liu SD, Zhao S, Natkunam Y, Nolan GP. Multiplexed ion beam imaging of
human breast tumors. Nat Med. 2014 Mar 2. doi: 10.1038/nm.3488. PubMed PMID: 24584119.
http://www.ncbi.nlm.nih.gov/pubmed/24584119
http://www.nature.com/nm/journal/vaop/ncurrent/full/nm.3488.html  (advanced online)

Giesen C, Wang HA, Schapiro D, Zivanovic N, Jacobs A, Hattendorf B, Schüffler PJ, Grolimund D, Buhmann JM, Brandt S, Varga Z, Wild PJ, Günther D, Bodenmiller
B. Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2869.PubMed PMID: 24584193
http://www.ncbi.nlm.nih.gov/pubmed/24584193
http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.2869.html  (advanced online)
Page 7 of the giesen PDF:
Data and software availability.
All raw data, image source data, cell segmentation masks, algorithms and software (Supplementary Software)
for data processing and SPADE trees (Supplementary Data) are available at http://www.cytobank.org/bodenmillerlab/.
http://reports.cytobank.org/113/v1
1 um pixel size, 500x500 um area (5 um tissue section) in in 3.5 hours, 20 pixels per second (~250,000 pixels). See the paper for their plex.
For comparison, reference 8 (now GE's MultiOmyx) did 60plex by pairwise immunofluorescence in 30 days for one tissue microarray (most of the time each day being sample prep/incubations/washes).


Enjoy,

George

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George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/