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This is a great introduction to a question a wanted to ask to the SMLM
specialists (I did not find the answer in the above reference). I also
posted it on the Nanoscopy LinkedIn group, so I'm sorry for people that
will get it two times!

There are several formulas to estimate the lateral (XY) precision of
individual localization events in PALM/STORM microscopy. This goes from the
crude sigma(PSF)/√Nphotons to more elaborate formulas that includes
pixelation and background (such as Thomson & Webb Biophys J 2002, corrected
in Mortensen Nat Methods 2010). What I'm looking for is an equation that
allows to estimate the precision for the Z localization when using
astigmatism-based 3D PALM/STORM. The reference I have found where this
precision is studied theoretically (such as Badierostami Applied Phys Lett
2010, Shaevitz Int J Optics 2009) do not use a directly calculable formula.

I assume the precision depends on the photon number and the point on the Z
calibration curve with the respective X and Y PSF width. Is anyone aware of
such a formula?

Best Regards,

Christophe




2014-03-04 21:52 GMT+01:00 Alberto Diaspro <[log in to unmask]>:

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> To join, leave or search the confocal microscopy listserv, go to:
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> http://www.nature.com/nmeth/journal/v11/n3/full/nmeth.2843.html
>