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Hi Magali,

Hopefully you have a resonant scanner to complement your Piezo type 
stage(s). R.S. enables as fast scanning in XZ as in XY. 14 full frames 
per second on the Leica SP5 I used to manage in Miami ... I believe the 
new SP8 RS is even faster, and acquiring fewer rows enables higher 
speed, something like 100 fps for 50 rows (the SP5 also had a rotator 
mirror to change scanning orientation wrt specimen).

If you do not have R.S., carefully evaluate your acquisition parameters 
for XZ to get the performance you need. I suggest using reflection mode 
on a coverglass, that is, the air-coverglass-air interfaces, timelapse 
mode, to evaluate Z-stability (reflection gives a much brighter signal - 
and photostable - compared to fluorescence ... on LSM7x0's need to click 
a checkbox to enable bright reflection ... also, use pinhole 1 A.U.). 
Changing Z-range should not affect how the specimen looks. If there is a 
bidirectional Z option you can ask whether the surfaces are in the same 
pixels in each direction.

Please publish what you find. It sounds like Alby can use more 
manuscripts for M.R.T (page charges, not gold open access without more 
charges). Also consider Journal of Biomolecular Techniques, 
http://jbt.abrf.org/  -- "gold" (immediate) open access and no 
publication charge and Rich Cole or Claire Brown might be the light 
microscopy handling editors?.


Enjoy,

George

On 6/10/2014 3:47 AM, Magali Mondin wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
>
>
> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
> for specific XZ applications. As we have some issues to use them for these
> specific applications, we would like to have general experience feedbacks
> with these Zeiss piezo stages installed on scanning confocals.
>
>
>
> Particularly concerning the ease of use, re-positioning reproducibility to
> himself and to the z limits defined with the micro-z, lifetime of the
> system installed on a very used microscope…
>
>
>
> And of course we are particularly interested with any experience with Z
> studies, either in XYZ mode followed by orthogonal projections, or using
> the XZ mode!
>
>
>
> Thanks for your Help!
>
>
>
> Mag
>
>
>
> *Magali Mondin, Ph.D.*
>   ------------------------------
>
> *Plateforme d'imagerie PRISM*
> iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
> Parc Valrose
> 06108 NICE cedex 2
>
> * tel: +33 (0) 4 92 07 64 29 fax :+33 (0)4 92 07 64 32*
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>
>    


-- 



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/