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Thanks for your answers.

More precisely, when we talk about "used" microscope it means that we have
a lot of users on both systems (LSM 710 and LSM 780) and we wonder about
the resistance of the stage over time ;)
For our fast XZ applications (live dynamics), on both LSM 710 and LSM 780
driven by ZEN, we observe distorsions of the images when compared to XZ
acquisitions done with the microscope Z. Does anyone tried these kind of
acquisitions with the piezo stage mounted on either 710 or 780 microscopes?

Thanks!

Mag




2014-06-10 17:01 GMT+02:00 Dan Focht <[log in to unmask]>:

> *****
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Magali
>
>
> If it is a old "very used microscope" you might want to consider having
> all the lubricants in the stage and focusing system cleaned and replaced
> with fresh lubricants to prevent slow settling or lagging that occurs due
> to viscosity changes in old lubricants before relying on such a sensitive
> translation device.
>
> Dan
>
>
>
>
>
> On Jun 10, 2014, at 4:47 AM, Magali Mondin wrote:
>
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>
> Dear all,
>
>
>
> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
> for specific XZ applications. As we have some issues to use them for these
> specific applications, we would like to have general experience feedbacks
> with these Zeiss piezo stages installed on scanning confocals.
>
>
>
> Particularly concerning the ease of use, re-positioning reproducibility to
> himself and to the z limits defined with the micro-z, lifetime of the
> system installed on a very used microscope…
>
>
>
> And of course we are particularly interested with any experience with Z
> studies, either in XYZ mode followed by orthogonal projections, or using
> the XZ mode!
>
>
>
> Thanks for your Help!
>
>
>
> Mag
>
>
>
> *Magali Mondin, Ph.D.*
> ------------------------------
>
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> * tel: +33 (0) 4 92 07 64 29 fax :+33 (0)4 92 07 64 32*
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> ------------------------------
>
>
> Dan Focht
> Bioptechs, Inc.
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> Butler, PA 16002
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> P: (724)282-7145
> F: (724)282-0745
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>