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For fast xz scanning the hydraulic coupling due to the immersion fluid between the slide and objective can cause distortion especially if the slide is not well supported and/or the coverslip not well fixed to the slide. 

HTH

Mark


On 11/06/2014, at 10:43 am, Magali Mondin <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Thanks for your answers.
> 
> More precisely, when we talk about "used" microscope it means that we have
> a lot of users on both systems (LSM 710 and LSM 780) and we wonder about
> the resistance of the stage over time ;)
> For our fast XZ applications (live dynamics), on both LSM 710 and LSM 780
> driven by ZEN, we observe distorsions of the images when compared to XZ
> acquisitions done with the microscope Z. Does anyone tried these kind of
> acquisitions with the piezo stage mounted on either 710 or 780 microscopes?
> 
> Thanks!
> 
> Mag
> 
> 
> 
> 
> 2014-06-10 17:01 GMT+02:00 Dan Focht <[log in to unmask]>:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>> 
>> Magali
>> 
>> 
>> If it is a old "very used microscope" you might want to consider having
>> all the lubricants in the stage and focusing system cleaned and replaced
>> with fresh lubricants to prevent slow settling or lagging that occurs due
>> to viscosity changes in old lubricants before relying on such a sensitive
>> translation device.
>> 
>> Dan
>> 
>> 
>> 
>> 
>> 
>> On Jun 10, 2014, at 4:47 AM, Magali Mondin wrote:
>> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>> 
>> Dear all,
>> 
>> 
>> 
>> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
>> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
>> for specific XZ applications. As we have some issues to use them for these
>> specific applications, we would like to have general experience feedbacks
>> with these Zeiss piezo stages installed on scanning confocals.
>> 
>> 
>> 
>> Particularly concerning the ease of use, re-positioning reproducibility to
>> himself and to the z limits defined with the micro-z, lifetime of the
>> system installed on a very used microscope…
>> 
>> 
>> 
>> And of course we are particularly interested with any experience with Z
>> studies, either in XYZ mode followed by orthogonal projections, or using
>> the XZ mode!
>> 
>> 
>> 
>> Thanks for your Help!
>> 
>> 
>> 
>> Mag
>> 
>> 
>> 
>> *Magali Mondin, Ph.D.*
>> ------------------------------
>> 
>> *Plateforme d'imagerie PRISM*
>> iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
>> Parc Valrose
>> 06108 NICE cedex 2
>> 
>> * tel: +33 (0) 4 92 07 64 29 fax :+33 (0)4 92 07 64 32*
>> mail : [log in to unmask]*; *[log in to unmask]
>> ------------------------------
>> 
>> 
>> Dan Focht
>> Bioptechs, Inc.
>> 3560 Beck Rd.
>> Butler, PA 16002
>> www.bioptechs.com
>> P: (724)282-7145
>> F: (724)282-0745
>> [log in to unmask]
>> 

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

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