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Dear Shalin,

have you seen *BMC Biotechnology* 2014, *14*:36.
doi:10.1186/1472-6750-14-36?

Kind regards, jens


On Wed, Jun 4, 2014 at 5:15 PM, Shalin Mehta <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello listers,
> We are imaging zebrafish embryos with 10x and the embryos just about
> fit the field of view at the development stage of interest
> (4hpf-30hpf).
>
> Are there tried and tested methods for giving the embryos room to
> grow, while keeping them within the field of view?
>
> Embryos are mounted in agarose and we can make space in agarose pad
> near the tail when the embryos are older. But, identifying the growth
> axis at young stages (~4hpf) is difficult.
>
> All inputs appreciated,
> Thanks
>
> Assistant Research Scientist,
> Marine Biological Laboratory,
> 7 MBL Street, Woods Hole MA 02543, USA
>
> website: http://mshalin.com
> (office) Lillie 110, (ph) 508-289-7374.
>



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