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On Wed, 17 Dec 2014 16:38:32 -0500, Marcia Boulina <[log in to unmask]> 
wrote:
>I will need a standard set - 488, 555, 561, 594, maybe 633 range (users
>have not decided if they wanted the cube yet). All we image in UV is DAPI.
>But I thought that there is an array, and LEDs come in every flavour.

We have a Lumencor SpectraX on our TE2000 and we love it. We bought one new 
Chroma quad-band dichroic and emission filter set, as well as 4 separate single-
band emission filters for our emission filter wheel (although this latter set is not 
absolutely necessary).

The amazing thing is to be able to run color sequences at the frame rate of the 
camera (because the SpectraX accepts TTL triggering of each line independently). 
It is beautiful to see the rainbow of light flashing out of the scope at 20+ frames 
per second!

https://micro-manager.org/wiki/Hardware-based_synchronization

We use a ESio TTL box controlled by Micro-Manager and it works great. But you 
could use an Arduino and some wiring to accomplish the same thing for cheaper.

We haven't run into any issues with brightness: the SpectraX is bright enough for 
all our cell imaging experiments. Typically, we run it at 20% power. That said, I'm 
aware that the very bright peaks in an arc lamp spectrum (e.g. UV, 435, 546) 
aren't there in the LED spectra. So for FRAP or something, you may not be able to 
bleach as fast.

-Sam