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December 2014

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Confocal Microscopy List <[log in to unmask]>
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Michael Giacomelli <[log in to unmask]>
Date:
Wed, 3 Dec 2014 18:02:16 -0500
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Has anyone used these dyes?  How specific are they for DNA?  Molecular
probes makes them sound quite interesting for labeling nuclei:

https://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-stains.html

But there is very little information on them.  I am interested in
alternatives to acridine orange for live cell imaging that are a little
more blue shifted.  Right now I am using DAPI which works well, but the
diffusion time is a bit slow in thick tissues relative to acridine orange
and its a bit less DNA specific at higher concentrations. An acridine
variant that kept the high specificity and rapid diffusion of AO but was a
little blue shifted would be perfect.

Mike

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