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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Jeremy,
independent of the fixation itself, you should also have an eye on your
embedding medium. Hardening medium such as Mowiol or ProLong tends to
flatten the cells quite a bit, compared to non-hardening such as
Vectashield.
Steffen
Am 09.12.2014 11:52, schrieb Jeremy Adler:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Cells on coverslips tend to flatten during fixation.
> In LM the Z resolution is always poorer than in XY and shrinkage in Z exacerbates this mismatch.
> Does anyone have any suggestions for preventing or reducing shrinkage.
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
Mail room:
Marchioninistr. 15, D-81377 München
Building location:
Marchioninistr. 27, München-Großhadern
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