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December 2014

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Olympus FV1000 Laser Replacement / Upgrade
From:
Naomi Melamed-Book <[log in to unmask]>
Date:
Sat, 13 Dec 2014 18:24:03 +0200
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HI,

We have replaced a 543 with a 561, our Olympus rep here in Israel replaced
it (about 2 years ago) and we got new dichroic and filters we needed.

Naomi

Naomi Melamed-Book, Ph.D
Bio-Imaging Unit-Director
The Alexander Silberman Institute of Life Science
Edmond Safra Campus (G Ram)
The Hebrew University
Jerusalem 91904, Israel
phone: 972-2-6585453
Fax:972-2-6586448
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Oggie Golub
Sent: Friday, December 12, 2014 11:14 PM
To: [log in to unmask]
Subject: Olympus FV1000 Laser Replacement / Upgrade

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Hello all,

I was wondering if anyone on this board has any experience or relevant
information regarding the replacement and potential upgrade of lasers on
an Olympus FV1000 confocal.

Our FV1000 has a 543nm HeNe that needs to be replaced soon, and instead of
replacing it with another relatively weak (~1.5mW max) HeNe tube, I was
hoping to upgrade the system by installing a 3rd party 561nm laser diode,
as they are much cheaper, more powerful, and more abundant lasers than 543
HeNe's.

However, I am concerned that the new wavelength won't get diffracted and
launched into fiber by the AOTF, and I haven't been able to find any
options in the FV software that will allow me to tune the AO. So I am
curious if anyone here has replaced any of the lasers on their FV1000 with
a 3rd party laser, and if so, how you went about mounting it in the box
and tuning the AOTF to accept the new laser line.

Any and all help regarding this topic would be greatly appreciated!

Thank you,
-Oggie


Oggie Golub
-----------------------
University of Oregon
Institute of Molecular Biology
PhD Candidate

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