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On Wed, 26 Nov 2014 19:21:50 +0000, Andreas Bruckbauer 
<[log in to unmask]> wrote:

>The beam expander on the newer Olympus multiphotons is just that, it is 
motorized and makes sure the back aperture is filled when using different 
objectives. You can just define a new objective with the right magnification 
and NA to 'fool' it. This way it is also possible to underfill existing objectives.
>Best wishes
>Andreas 

At least on our single-photon Olympus FV1000 (software v. 3.1), there is a 
plain text file somewhere in the Fluoview installation folder that contains 
the list of objectives. You can edit the file to add definition of another 
objective. If I remember correctly, it contains information like 
magnification, NA, immersion medium, working distance, back aperture 
diameter, ..
I did that when testing a Zeiss 25x/0.8  multiimmersion objective on the 
Olympus  (worked, but as expected had a pretty significant chromatic 
aberration). I do not remember the name of the definition file.

Stan Vitha
Texas A&M University
Microscopy and Imaging Center