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Hi, Alex,

Alex,

How closely can you incise the points?  1um apart?  Less?

Jason



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Alex Corbett
Sent: Friday, February 27, 2015 9:05 AM
To: [log in to unmask]
Subject: Re: recommended distortion target for multiphoton?

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I have recently joined the confocal microscopy list, so apologies if this post is not reaching the correct people. I am following up on a thread I was forwarded by a colleague from September
2014 regarding targets for determining distortion over an imaging volume. We have been trialling a new solution which I have found particularly useful for sensitive measurements of distortion within an image volume. 

The solution is a fluorescent slide in which a 3D fabrication pattern has been laser written. The fabrication points appear dark in single and two-photon fluorescence. There is the flexibility to write any pattern although I have so far been using a columnar pattern on a 10um square grid. The plastic slides are very robust and come in a range of fluorophores. If you think this approach might be useful, you can find more details by first downloading the (open access) paper describing it here: 
http://journal.frontiersin.org/article/10.3389/fphys.2014.00384/abstract

If you would like to try it for yourself then please contact me and we will see about arranging a free trial. 

Best regards, Alex.