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April 2015

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Subject:
multi-photon vs near-IR one photon microscopy
From:
Vitaly Boyko <[log in to unmask]>
Reply To:
Vitaly Boyko <[log in to unmask]>
Date:
Thu, 9 Apr 2015 03:36:02 +0000
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Dear Members,it is a kind of known that multi-photon microscopy is "smart" on the excitation and "dumb" on the emission side. To challenge my "over flattened bimodal exaggeration" has someone compared performance of the one photon microscopy in the near-IR portion of spectra (with near-IR dye emission max in the range of 750-950 nm) with multi-photon microscopy of, let say, EGFP/Alexa 488 in sensitivity (in pico-nano Molar range, hopefully), SNR, photo-toxicity, emission light scattering, etc.?Any reference would be greatly appreciated.
Thank you.
Vitaly
   
   

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