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Good luck!

Martin Wessendorf



On 4/9/2015 11:15 AM, Kubow, Kristopher E - kubowke wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I'd like to know if anyone has a protocol for preparing and using the stain Uvitex (also known by many other names: http://www.chemicalbook.com/CASEN_7128-64-5.htm). I have a researcher who ordered it to stain crayfish intestines. However it came as a solid powder rather than a solution, and without any instructions. I've never heard of the stain before and there is very little obvious information out on the internet. Based on its chemical structure, I'm thinking that they need to make a concentrated stock solution in a non-polar solvent such as DMF or DMSO. But I'd like to know if anyone has a standard protocol for this.
>
> Thank you,
>
> Kristopher Kubow
> Assistant Professor
> Director of the Light Microscopy Facility
> Department of Biology
> James Madison University
> Harrisonburg, VA 22807
> (540) 568-4521
> Bioscience 2036
> http://csmbio.csm.jmu.edu/biology/microscopy/

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