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April 2015

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Subject:
Removing DAB IHC staining
From:
Paul Rigby <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 1 Apr 2015 13:08:07 +0800
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Hi All,
One of our users has had a mishap with some very precious tumour sections which were accidentally stained with the wrong primary antibody followed by DAB. They want to know if it is possible to destain the sections (remove the DAB, which is, apparently, quite dark). They have experience removing immunofluorescence antibody staining but not the DAB product.
All suggests will be very gratefully accepted.
Many thanks
Paul

Assoc. Prof. Paul Rigby
Optical Microscopy Specialist
Centre for Microscopy, Characterisation & Analysis (M510)
The University of Western Australia
35 Stirling Highway
Crawley  WA  6007
Australia

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