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Hi Christian,
we have done SIM of GFP-tagged cytoskeletal protein in Arabidopsis 
chloroplasts, but not in live tissue. I vacuum infiltrated the tissue with 3% 
formaldehyde and then fixed using a microwave processor. Afterwards  
washed, infiltrated gradually with glycerol-based mountant (80% glycerol 
buffered with p-penylenediamine, if I remember correctly) again assisted 
with microwave irradiation to speed it up.

It worked quite well; The fluorescence held up well enough to survive 
having the slide put in a mailer and shipped off for imaging (no 
refrigeration).
If I was doing it again I would use one of those high-index mounting media 
(not thiodiethanol, but there are some glycerol-based commercial products 
with R.I. 1.52)  to reduce spherical aberration.

Stan Vitha
Microscopy and Imaging Center
Texas A&M University