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Hi Christophe and Professor Xi,

The images are of fluorescent beads, which should have many different 
fluorescent labels attached to their surfaces with dipoles pointing in 
many different directions. I don't think that any single dipolar 
emission pattern should be obvious since the patterns from all the other 
labels on the same sphere should average out, but I could be wrong about 
this.

I've also seen this same aberration in images of 100 nm fluorescent 
beads on a custom microscope with multiple NA >= 1.45, 100x oil 
immersion objectives. No degree of alignment of the imaging pathway 
seems to fully correct it, at least in my hands. In the end, I also 
suspected coverslip tilt based on the Arimoto paper and this 
dissertation from Stefan Hell's lab: 
https://ediss.uni-goettingen.de/bitstream/handle/11858/00-1735-0000-000D-F0B1-E/berning.pdf?sequence=1

Best,
Kyle

On 04/21/2015 03:01 PM, Peng Xi wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Christophe,
>      Is your TIRF rotational polarization or linear polarization? It seems
> to me like a fluorescence dipole PSF.
>

-- 
Kyle M. Douglass, PhD
Post-doctoral researcher
The Laboratory of Experimental Biophysics
EPFL, Lausanne, Switzerland
http://kmdouglass.github.io
http://leb.epfl.ch