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Hi Gabor

We do a fair bit of Cars at the IGMM. Can I ask you to send me a personal email and I can put you in touch with the people who are doing it. We use CARs on a multiphoton system and I'm sure can advise about appropriate lasers.

Best wishes

Ann


Dr Ann Wheeler
Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU
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T: 0131 651 8665
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Csúcs Gábor
Sent: 23 June 2015 11:37
To: [log in to unmask]
Subject: CARS imaging on a scanning confocal

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Dear All,

For a user project we would like to establish CARS imaging on one of our scanning confocals (more precisely on a multi-photon system). Unfortunately from the "big fours" (according my information) only Leica offers a commercial solution for CARS but they use a special laser (which for various reasons is not an option for us). Our idea would be to use a "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual wavelength option) as light source. Ideally we would install this modality on a Zeiss LSM multiphoton system. My question is hence twofold:
1) Is there anyone who has established CARS imaging on a Zeiss confocal?
2) Is there anyone who has used the above mentioned laser for realizing CARS?

I would very much appreciate any feedback, experience report.

Thanks           Gabor