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COMMERCIAL RESPONSE

Dale,

In case the link didn't go through properly, I'll try again:

https://www.chroma.com/sites/default/files/5-CHANNEL%20FLUORESCENCE%20IMAGING%20SIMPLIFIED%20-%20Reliable%20Multiplexing%20for%20the%20Non-Specialist_New.pdf

Jeff


*Jeff Carmichael*

*Technical and Product Marketing Manager*

*[log in to unmask] <[log in to unmask]>*Chroma Technology Corp.

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On Thu, Feb 16, 2017 at 11:12 AM, Dale Moulding <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
> does anyone know of a way to irreversibly destroy alexa fluor fluorescence
> so a sample can be stained with a second set of dyes?
> We do whole mount staining for 3 antigens with Alexa 488, 568 and 633. We
> would then like to re-image the same samples with cellmask and phalloidin.
> Is there any way to kill alexa fluor fluorescence without destroying
> membranes and phalloidin binding?
> Cheers
> Dale
>