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February 2017

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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"Feinstein, Timothy N" <[log in to unmask]>
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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 8 Feb 2017 16:36:53 +0000
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Hi all, 

Have people with spinning discs compared the Photometrics 95b against leading EM-CCD and sCMOS options?  I am interested to know how it compares in real world use, especially: 

• Sensitivity, taking into account that larger pixels might need more C-mount magnification to reach Nyquist resolution
• Does non-uniform pixel noise inherent to sCMOS complicate your quantifications?  This seems like an bigger deal in low light situations where a 95% QE sensor would be most desirable.  
• How are you controlling it – Metamorph, Micro-Manager, or something else?  Some major scope makers still do not have driver support.   

I have heard that other manufacturers will release their own back-thinned sCMOS cameras soon.  It will be nice to have some competition in that category.  

No commercial interest.  Thanks! 


Tim
	
Timothy Feinstein, Ph.D. 
Research Scientist
University of Pittsburgh Department of Developmental Biology 


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