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Well, I used Hoechst once to stain nuclei in a tissue. Despite cleaning 
of the dipping objective, we did see nuclear staining in subsequent 
experiments. So yes, the lens may carry over a dye. Whether or not this 
is a problem depends, of course. At the time it didn't bother us too 
much and eventually the effect was lost.

It might help to keep the lens in a large water bath for some hours 
(with flow?), to speed up the wash out.

Steffen


Am 15.02.2017 um 04:20 schrieb Claire Brown, Dr.:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
> Would this be a problem for the dipping lenses?
> I would assume as long as the lens is cleaned well after use it should be fine.
>
> Anyone think otherwise?
>
> Sincerely,
>
> Claire
>

-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de