CONFOCALMICROSCOPY Archives

February 2017

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
destroy alexa fluor fluorescence to allow restaining
From:
Dale Moulding <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 16 Feb 2017 10:12:43 -0600
Content-Type:
text/plain
Parts/Attachments:
text/plain (13 lines) 
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,
does anyone know of a way to irreversibly destroy alexa fluor fluorescence so a sample can be stained with a second set of dyes? 
We do whole mount staining for 3 antigens with Alexa 488, 568 and 633. We would then like to re-image the same samples with cellmask and phalloidin. 
Is there any way to kill alexa fluor fluorescence without destroying membranes and phalloidin binding?
Cheers
Dale

ATOM RSS1 RSS2