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March 2022

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From:
"Kilgore, Jason A." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 4 Mar 2022 16:55:09 +0000
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*****
To join or leave the confocal microscopy listserv or to change your email address, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Along the same vein of discussion, I thought this would be a good place to post links to the many different spectra viewer tools online that I've found:


Thermo Fisher Scientific SpectraViewer:
https://www.thermofisher.com/order/fluorescence-spectraviewer#!/

Omega Filter’s Curvomatic:
https://www.omegafilters.com/curvomatic

Max Planck Institute Spectra Viewer:
https://public.brain.mpg.de/shiny/apps/SpectraViewer/

Chroma’s Spectra Viewer:
https://www.chroma.com/spectra-viewer

FP Base (fluorescent proteins only):
https://www.fpbase.org/spectra/

Semrock’s Searchlight:
https://searchlight.semrock.com/

Fluorophores.org (click on dye to see spectra):
http://www.fluorophores.tugraz.at/substance/

BioLegend Spectra Analyzer:
https://www.biolegend.com/spectraanalyzer


Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Stephan Junek
Sent: Friday, March 4, 2022 7:26 AM
To: [log in to unmask]
Subject: Re: New spectra viewer

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [log in to unmask]


*****
To join or leave the confocal microscopy listserv or to change your email address, go to:
https://urldefense.proofpoint.com/v2/url?u=https-3A__lists.umn.edu_cgi-2Dbin_wa-3FSUBED1-3Dconfocalmicroscopy-26A-3D1&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=Y_2CEfTPdWSetElgPgSThaW-uLEpH07DY8LCWh4vz-0&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=f0fo-TXErTGMokMyHvCLYo7aIX020bbDb4gncKgQhUM&e=  and include the link in your posting.
*****

Dear all,

thanks for checking the viewer. The error messages reported by several people were due to overload of our server, this problem should now be resolved. But please let me know if you still encounter problems.

Best wishes

Stephan

Am 03.03.2022 um 09:03 schrieb Arnim Jenett:
> *****
> To join or leave the confocal microscopy listserv or to change your 
> email address, go to:
> https://urldefense.proofpoint.com/v2/url?u=https-3A__lists.umn.edu_cgi
> -2Dbin_wa-3FSUBED1-3Dconfocalmicroscopy-26A-3D1&d=DwIDaQ&c=q6k2DsTcEGC
> cCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-y
> ioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteL
> dLIe5&s=Y_2CEfTPdWSetElgPgSThaW-uLEpH07DY8LCWh4vz-0&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=f0fo-TXErTGMokMyHvCLYo7aIX020bbDb4gncKgQhUM&e=  and include the link in your posting.
> *****
>
> I get the same error as reported last night by Craig.
> The GUI is rendered but behind a grey transparent overlay. Cursor 
> turns into a no-parking sign and in the left lower corner hovers the 
> error message "Disconnected from the server. Reload" which reloads the 
> same page/error, when clicked on.
> Database overwhelmed?
> bon courage,
> aRnim
>
> On 22/03/02 19:47, Craig Brideau wrote:
>> *****
>> To join or leave the confocal microscopy listserv or to change your 
>> email address, go to:
>> https://urldefense.proofpoint.com/v2/url?u=https-3A__lists.umn.edu_cg
>> i-2Dbin_wa-3FSUBED1-3Dconfocalmicroscopy-26A-3D1&d=DwIDaQ&c=q6k2DsTcE
>> GCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQp
>> V-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cD
>> RteLdLIe5&s=Y_2CEfTPdWSetElgPgSThaW-uLEpH07DY8LCWh4vz-0&e=
>> Post images on 
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=f0fo-TXErTGMokMyHvCLYo7aIX020bbDb4gncKgQhUM&e=  and include the link in your posting.
>> *****
>>
>> I went to take a look, but am getting "disconnected from the server"
>> messages and the entire page is greyed out.
>> Hopefully just a transient internet issue.
>> Craig
>>
>> On Wed, Mar 2, 2022 at 8:39 AM Stephan Junek 
>> <[log in to unmask]>
>> wrote:
>>
>>> *****
>>> To join or leave the confocal microscopy listserv or to change your 
>>> email address, go to:
>>> https://urldefense.proofpoint.com/v2/url?u=https-3A__lists.umn.edu_c
>>> gi-2Dbin_wa-3FSUBED1-3Dconfocalmicroscopy-26A-3D1&d=DwIDaQ&c=q6k2DsT
>>> cEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7sr
>>> QQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYX
>>> l-cDRteLdLIe5&s=Y_2CEfTPdWSetElgPgSThaW-uLEpH07DY8LCWh4vz-0&e=
>>> Post images on 
>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=f0fo-TXErTGMokMyHvCLYo7aIX020bbDb4gncKgQhUM&e=  and include the link in your posting.
>>> *****
>>>
>>> Dear all,
>>>
>>> I would like to draw your attention to our new fluorophore database 
>>> and spectra viewer. You might be wondering why we need yet another 
>>> spectra viewer, but I think we included some very useful features:
>>>
>>>    * The viewer uses a large database with more than 550 
>>> fluorophores and
>>>      spectra of more than 250 optical elements (filters and mirrors).
>>>    * The database contains two- and three-photon excitation spectra for
>>>      more than 150 and 10 fluorophores, respectively. We are regularly
>>>      adding additional spectra. Please let us know if you have spectra
>>>      that are currently not included.
>>>    * Custom spectra can be uploaded and displayed along with the 
>>> spectra
>>>      contained in the database.
>>>    * Plots can be displayed using normalized or absolute values on a
>>>      linear or logarithmic axis.
>>>    * In addition to the “standard” spectral plots the app provides a 
>>> “2D
>>>      viewer” in which excitation and emission spectra are combined into
>>>      2D contour plots. This is particularly useful when displaying
>>>      excitation/emission spectra for various fluorophores 
>>> simultaneously.
>>>    * A large number of additional fluorophore properties are 
>>> contained in
>>>      the database and can be accessed through a fluorophore browser (a
>>>      table with various search and filter functions) or visualized 
>>> using
>>>      a 2D scatter plot. Up to four properties can be visualized
>>>      simultaneously using the marker’s x- and y-coordinates as well as
>>>      their size and color.
>>>    * The viewer works quite well on mobile devices.
>>>
>>> You can find the viewer here:
>>> https://urldefense.proofpoint.com/v2/url?u=https-3A__public.brain.mp
>>> g.de_shiny_apps_SpectraViewer_&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8
>>> hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=Ep
>>> EjaXITnXbitVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=Gs3
>>> mnsTKiYeDHv4h17xm8L54DCabBOHNFLOtEWpmHcY&e=  and additional 
>>> information here: 
>>> https://urldefense.proofpoint.com/v2/url?u=https-3A__brain.mpg.de_32
>>> 6043_spectra-2Dviewer&d=DwIDaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH
>>> 8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=EpEjaXITnXb
>>> itVaDvZ2OZfWd0J8bKXWK56Hom_PGcUzZQUoDKYXl-cDRteLdLIe5&s=8UDBBI0R6X62
>>> -SJnhozRNIZQipjp7fpf27XqMZDsH2M&e=
>>>
>>> I would be happy to get your feedback (questions, comments, bug 
>>> reports, feature requests …).
>>>
>>> Best wishes,
>>>
>>> Stephan.
>>>
>>> --
>>> ________________________________________
>>>
>>> Dr. Stephan Junek
>>> Head of the imaging facility
>>> Max Planck Institutes for Brain Research and Biophysics 
>>> Max-von-Laue-Str. 4
>>> 60438 Frankfurt am Main
>>> Germany
>>>
>>> [log in to unmask]
>>> T: +49 69 850033 – 2531 (Office)
>>> T: +49 69 850033 – 1409 (Lab)
>>> F: +49 69 850033 – 2102
>>>
>
--
________________________________________

Dr. Stephan Junek
Head of the imaging facility
Max Planck Institutes for Brain Research and Biophysics Max-von-Laue-Str. 4
60438 Frankfurt am Main
Germany

[log in to unmask]
T: +49 69 850033 – 2531 (Office)
T: +49 69 850033 – 1409 (Lab)
F: +49 69 850033 – 2102

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