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April 2024

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Subject:
From:
Marko Lampe <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 30 Apr 2024 12:03:51 -0500
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Dear all,

We would like to draw your attention to the Microscopy Course “Time-resolved STED nanoscopy in life sciences” at EMBL Heidelberg (Germany) from July 8th to 13th.

This six-day comprehensive advanced microscopy course focuses on stimulated emission depletion microscopy (STED) with a special focus on lifetime-based STED and its application to biological questions.

The following topics will be covered in lectures and tutorials as well as in practical workshops:

-    Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
-    Technical implementations – pulsed-STED, gated-STED and time-resolved STED (tauSTED)
-    Multi-colour 2D and 3D STED microscopy
-    Image acquisition strategies for live-cell STED
-    STED multiplexing via lifetime-based fluorophore separation
-    Clearing for large volume STED imaging
-    Expansion-microscopy STED
-    Deconvolution of time-resolved STED data
-    Image analysis

Participants will have the opportunity for exercises on state-of the art STED microscopes and will acquire high quality nanoscopy data during the course.

For more information, please have a look at:
https://www.embl.org/about/info/course-and-conference-office/events/mic24-03/

PLEASE NOTE: Application deadline is already May 6th!

The course will be supported by Leica Microsystems.

On behalf of the organizing committee,
Marko

-- 
_____________________________
Dr. Marko Lampe
Advanced Light Microscopy Facility
EMBL Heidelberg
Meyerhofstr.1
D - 69117 Heidelberg
Phone +49(0)6221 387-8884
www.embl.de/almf
www.embl.org/elmi

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