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Date: | Tue, 30 Apr 2024 12:03:51 -0500 |
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Dear all,
We would like to draw your attention to the Microscopy Course “Time-resolved STED nanoscopy in life sciences” at EMBL Heidelberg (Germany) from July 8th to 13th.
This six-day comprehensive advanced microscopy course focuses on stimulated emission depletion microscopy (STED) with a special focus on lifetime-based STED and its application to biological questions.
The following topics will be covered in lectures and tutorials as well as in practical workshops:
- Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
- Technical implementations – pulsed-STED, gated-STED and time-resolved STED (tauSTED)
- Multi-colour 2D and 3D STED microscopy
- Image acquisition strategies for live-cell STED
- STED multiplexing via lifetime-based fluorophore separation
- Clearing for large volume STED imaging
- Expansion-microscopy STED
- Deconvolution of time-resolved STED data
- Image analysis
Participants will have the opportunity for exercises on state-of the art STED microscopes and will acquire high quality nanoscopy data during the course.
For more information, please have a look at:
https://www.embl.org/about/info/course-and-conference-office/events/mic24-03/
PLEASE NOTE: Application deadline is already May 6th!
The course will be supported by Leica Microsystems.
On behalf of the organizing committee,
Marko
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_____________________________
Dr. Marko Lampe
Advanced Light Microscopy Facility
EMBL Heidelberg
Meyerhofstr.1
D - 69117 Heidelberg
Phone +49(0)6221 387-8884
www.embl.de/almf
www.embl.org/elmi
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