In message <[log in to unmask]> ANDREW VAILLANT writes:
> We are opertaing a LEICA confocal here at the department of biology at the
> univ
> ersity of Ottawa.  I am interested in doing triple labelling using CY5 but I
> la
> sk the right filter cube to do this.  Could anybody out there tell me which
> bea
> n splitter and band pass filter are optimal for collecting light emitted by
> CY5
 
I don't know exactly what Leica offers, but if you're using a Kr/Ar-ion laser,
you'd want something like a 642-652 nm bandpass exciter filter (to isolate the
red line of the laser), a 660 nm dichroic mirror, and a 670 nm longpass barrier
filter to block the laser light from the PMT.
 
Hope that helps--
 
Martin Wessendorf