Re> Andrew Vaillants question concerning CY5 imaging in Leica CLSM:
 
Andrew,
 
there are two options for triple labelled specimens in the Leica CLSM:
 
1. You get a triple dichroic 488/568/647 as main beam splitter and
   a longpass filter 665 as CY5 barrier filter. This allows for
   sequential and simultaneous imaging and certainly is the most
   flexible choice. The drawback is a sligthly reduced efficieny
   for green and red emission [not deep red CY5 emission] due
   to the necessity to have barrier filters of appropriate bandwidths
   to get no reflections of the higher lines into the image.
 
2. For pure sequential imaging, we can provide a 660nm dichroic
   with a longpass 665 nm for the CY 5 detection.
 
Both filter combinations are standard Leica parts.
 
Best regards
 
Martin
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Martin Hoppe. Ph.D.       E-mail:
Leica Lasertechnik GmbH   Office: [log in to unmask]
Im Neuenheimer Feld 518   Home:   [log in to unmask]
D 69120 Heidelberg
Germany
Phone: +49 6221 41480
Fax:   +48 6221 414833
 
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