Does anyone have any advice or experience with loading cells (esp cardiac
myocytes) with sodium green for sodium measurements. I have tried various
loading procedures (with and without pluronic, diff concentrations and
temperatures) but only get a very faint signal in resting myocytes. When
exposed to conditions that should cause a rise in intracellular sodium
(gramicidin, hypoxia, ouabain) there is no change in fluorescence. The
fluorophore is being excited with the 488 line on a MRC600 and imaged at
around 530nm.
 
Any ideas ?
 
Ian.