Hello John,
 
I include in my mounting medium 5% n-propyl-gallate (Sigma) in a 1:1
glycerol:Phosphate buffer saline mixture.  I have found this to work well
with FITC and TRITC fluorochromes.  The anti-oxidant takes time to
dissolve at room temperature, so I make it up the night before I need to
use it.
 
Wilfred Denetclaw Jr., Ph.D.
 
 
On Fri, 27 Jan 1995, John G. Aghajanian, Ph.D. wrote:
 
> Hello folks,
>
> This may sound somewhat trivial but as one just starting out confocaling it
> is somewhat pertinent.  Is there a single anti-fade agent that works best
> for many/all fluorophores or are there certain anti-fade agents that work
> best with particular fluorophores? And, what are they?
>
> Thanks in advance,
>
>  ___________________________________________________
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>  |   John G. Aghajanian, Ph.D.                     |
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>