You can do this but,  you  will lose 50 % of your texas red signal, as the
major portion of this signal is between 610 and 625.  You would be better
off  with a 610 or no more than 615 long pass.
 ----------
From: owner-confocal
To: Multiple recipients of list CONFOCAL
Subject: 625nm long pass for Biorad 600 A2 to reduce bleed
Date: Thursday, August 10, 1995 11:26AM
 
Has anyone replaced the 600nm long pass filter in the A2 block with a
longer wavelength cutoff?  This would reduce the bleed of the red end
of the FITC emission through the red filter.  It seems as if a 625nm
cutoff would be appropriate and would not sacrtifice too much of the
red emission fro texas red etc.  Has anyone tried this?  How were the
results?  From whom was the filter purchased?  Omega Optical can
supply one on special order and said it should be polished and AR coated
for imaging applications.  Has anyone purchased a suitable part from another
company?
Thanks.
 
Milton Charlton
University of Toronto