>We have been debating the pros and cons of an inverted against an
>upright microscope for confocal applications.
>
>Are there any experiments that can be done ONLY using an upright ?
>
In brain slices we have been unable to do any experiments involving impaling
cells from above for intracellular recording etc while observing from below.
 
An inverted microscope is also not the best configuration for any slice
experiments as the cells on the bottom of the slice have the least access to
medium, oxygen, drugs etc.
JOHN
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John Cork
Calcium Imaging Facility,
LSUMC, New Orleans.
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