CONFOCALMICROSCOPY Archives

August 1995

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Confocal Microscopy List <[log in to unmask]>
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Thu, 24 Aug 1995 12:25:01 -1100
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>>Confocal folk:
>>We are reconstructing the body-wall musculature in lower worms
>>  using BODIPY/Phalloidin and confocal.
>>So far, we have no trouble importing the MRC-1000 files to NIH Image
>>  and creating both stacks and projections (Thanks, Harvey Karten!),
>>  which are extremely informative, as long as they're animated.
>>We are now to the point of wanting to make "pretty pictures" of stills for
>>   for seminars. If  I want a rendered, shaded reconstruction, do I
>>   have any alternatives other than  a SGI and Voxel View
>>   (financially out of the question).  I'm aware of VolVis,
>>  but how do I get from the confocal image stack to voxel data?  Is there a
>>  standalone program that does the necessary interpolation between image
>>  planes in the stack?
>>TIA
>>Julian Smith III
>
>Voxel View will run on Macs as well and the Mac version is much cheaper than
>the Silicon Graphics one.
>
>Vaytek have a package caled VoxBlast which is available for PC or Mac
>(and Unix, at a higher price).  I use an old version of Voxblast on my
>personal PC and have VoxelView on a Silicon Graphics which is used by
>all the many users of our lab.  I find both useful.
>
>The one drawback with PC or Mac versions of both packages is that they
>use dongles for copy protection, and in my experience these are very
>unreliable devices, prone to failure and also liable to have unforeseen
>side effects on computer operation.  It is very annoying to have a
>$1000 piece of software unusable because of a device which is only
>there because the vendor doesn't trust me.
>
>                                Guy Cox
>                                [log in to unmask]
>

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