CONFOCALMICROSCOPY Archives

August 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
autofluorescent blood vessels
From:
Judy Drazba <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 28 Aug 1995 20:19:29 EDT
Content-Type:
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text/plain (19 lines) 
Can someone out there can help me get rid of, or at least reduce the
autofluorescence from blood vessels in our sections.  We do all of our
imaging on brain slices (10-100 microns) fixed in 4% Paraform./PBS. I've
tried various blocking buffers and routinely include glycine to help quench
aldehydes to no avail.  Any suggestions??
 
Thanks,
Judy Drazba
 
Judy Drazba, Ph.D.  ([log in to unmask])
(Director, Confocal Microscopy Facility)
Department of Neurosciences, NC-3
The Cleveland Clinic Foundation
9500 Euclid Avenue
Cleveland, OH 44195-5001
Office (216)445-3760
Lab    (216)444-8712
FAX    (216)444-7927

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