On Fri, 15 Sep 1995, Erik Manders wrote:
 
> Try to avoid sequential scanning!
> It induces positional shift between the red and the green
> image. If you scan both colours simultaneously, you will
> have also some shift (50-200 nm) but you can correct for
> that because it is for all recordings the same. Re-scanning,
> however, induces unpredictable shift, so, correction is not
> possible.
 
I would like to add here that the shift during simultaneous acquisition
appears to be axial chromatic error, which is usually introduced by the
objective lens. Therefore, recalibration is needed for each lens.
 
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Stamatis Pagakis
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