In the general topic of optional laser lines for minimizing
FITC bleeding into the red channel, it should be noted that
some argon lasers (such as the Omnichrome model provided with
Noran systems) have a 529 nm line which is much better than
the 514 line. Unless the FITC signal is _very_ strong, there
is no cross-excitation of FITC by the 529 line.
 
Also, for some experiments requiring TRITC (where LRSC or
Texas Red can't be used), we've found that using the 474/476 nm
doublet of the Omnichrome laser to be a better line than the
standard 488 nm line for double labeling. The 488 nm line
is still on the upward slope of the TRITC excitation profile
while the 474/476 line is at the bottom of the tail.
 
daniel chin
research scientist, agouron institute
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