CONFOCALMICROSCOPY Archives

September 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Paul Goodwin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 22 Sep 1995 11:36:39 -0700
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I discovered a bit more than a year ago that the fluorescent looking
plastic that is often used in kids toys and for signs is wonderfully
fluorescent and stable. If you can't lacate any (try a plastics
manufacturer in your town) let me know and I'll secure a chunk for you.
 
________________________________________________________________________________
 
 
Paul Goodwin
Image Analysis Lab
FHCRC, Seattle, WA
 
On Fri, 22 Sep 1995, Finn-Mogens Haug wrote:
 
> Dear list,
>
> (I hope this is not too far off topic, but) we have looked mostly
> in vain for such standards, specifically "non-bleaching", inorganic,
> specimens suitable for 1/ routinely checking excitation level and for
> 2/ correcting spatial uniformities of the optical/digital imaging system.
>
> Reports and handbooks describe the use of uranyl-glass and inorganic
> crystals, but the only commercial product we have found contains a
> 1-2 mm circular fluorescing area of unknown composition, mounted on a
> microscope slide. Price: USD 1400 incl VAT. Expensive?
>
> A local contact makes Yttrium-Oxy-Sulfide crystals with several discrete
> emission/excitation bands for use in microspectrofluorimetry. However,
> the powder's granularity may preclude its use in de-calibrating
> pixel-to-pixel non-uniformities.
>
> Names, fax/phone-numbers and e-mail adresses of sources of such standards
> would be most helpful, perhaps to others as well.
>
>
> Thanks in advance -
>
>
>
>
> Finn-Mogens Haug
>
> Department of anatomy                   E-mail: [log in to unmask]
> Institute of basic medical sciences     Phone : +47 22 85 12 67
> University of Oslo                      Fax   : +47 22 85 12 78
>

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